Journal: World Journal of Surgical Oncology

Article Title: Circ_0001741 regulates proliferation and invasion in ESCC via the miR-194-5p/E2F3 axis

doi: 10.1186/s12957-025-04124-2

Figure Lengend Snippet: E2F3 is a miR-194-5p target gene in TE2 and KYSE150 cells A Predicted partially complementary binding sequence between E2F3 mRNA and miR-194-5p B Dual-luciferase reporter assays confirming miR-194-5p binding to the E2F3 3’UTR. C Pull-down assay was used to confirm the interaction of E2F3 and miR-194-5p D Western blot analysis of E2F3 protein levels in TE2 cells after miR-194-5p overexpression. * P < 0.05

Article Snippet: Membranes were incubated overnight at 4 °C with E2F3 primary antibodies (Proteintech, China, Cat No. 27615-1-AP), followed by HRP-conjugated secondary antibodies (Proteintech, China).

Techniques: Binding Assay, Sequencing, Luciferase, Pull Down Assay, Western Blot, Over Expression

Journal: World Journal of Surgical Oncology

Article Title: Circ_0001741 regulates proliferation and invasion in ESCC via the miR-194-5p/E2F3 axis

doi: 10.1186/s12957-025-04124-2

Figure Lengend Snippet: E2F3 overexpression reverses the effects of circ_0001741 knockdown A E2F3 overexpression partially rescues the anti-invasive effects of si-circ_0001741 or miR-194-5p in TE2 and KYSE150 cells B E2F3 overexpression partially restores proliferation suppressed by si-circ_0001741 or miR-194-5p. * P < 0.05

Article Snippet: Membranes were incubated overnight at 4 °C with E2F3 primary antibodies (Proteintech, China, Cat No. 27615-1-AP), followed by HRP-conjugated secondary antibodies (Proteintech, China).

Techniques: Over Expression, Knockdown

Journal: Frontiers in Oncology

Article Title: Identification of MAD2L1 as a novel biomarker for hepatoblastoma through bioinformatics and machine learning approaches

doi: 10.3389/fonc.2025.1524714

Figure Lengend Snippet: MAD2L1 activates E2F transcription and regulates cell cycle regulators. (A, B) E2F transcription factor relative luciferase activity in dual-luciferase reporter gene assay. (C, D) The effect of MAD2L1 knockdown on Cyclin A2, E2F3 and Cyclin E1 expressions was detected by western blotting. (E, F) The effect of MAD2L1 knockdown on cell cycle was explored using a flow cytometry assay. *p < 0.05, **p < 0.01, ***p < 0.001, p ****<0.0001.

Article Snippet: Primary antibodies against MAD2L1 (1:600, Cat. No. 15283-1-AP, Proteintech), β-ACTIN (1:10,000, Cat. No. N901r, CellGene), β-Tubulin (1:1,500, Cat. No. E-AB-40518, Elabscience), GAPDH (1:3000, Cat. No.AF7021, AFFINITY), N-Cadherin Rabbit mAb (1:1000, Cat. No. A19083, ABclonal), E-Cadherin Rabbit mAb (1:1000, Cat. No. A20798, ABclonal), Cyclin A2 Polyclonal antibody (1:10000, Cat. No. 18202-1-AP, Proteintech), PCNA Polyclonal antibody (1:5000, Cat. No. 10205-2-AP, Proteintech), CyclinE1(1:1000, Cat. No. 11554-1-AP, proteintech) and E2F3(1:1000, Cat. No. 27615-1-AP, proteintech) were incubated overnight at 4°C.

Techniques: Luciferase, Activity Assay, Reporter Gene Assay, Knockdown, Western Blot, Flow Cytometry

Journal: Cell reports. Medicine

Article Title: Signaling-induced systematic repression of miRNAs uncovers cancer vulnerabilities and targeted therapy sensitivity.

doi: 10.1016/j.xcrm.2023.101200

Figure Lengend Snippet: Figure 4. Drug prediction by miRNA sequencing uncovers drug sensitivities (A) Heatmap and unsupervised clustering according to the miRNA expression pattern of 12 included cancer models. (B) Overview of predicted activated pathways and corresponding PRSs for analyzed samples. The most frequent active pathways include WNT, TGF-b, and neurotophin (MAPK). (C) Overview of the top predicted target genes that are part of KEGG pathways. The most frequently predicted activated target genes are the cancer-associated genes E2F3 and CDK6.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Polyclonal anti-E2F3 antibody Proteintech Cat# 27615-1-AP; RRID: AB_2880926 CDK6 (D4S8S) Rabbit mAb Cell Signaling Technology Cat# 13331; RRID: AB_2721897 Activin Receptor Type IIA antibody Biozol Cat# GTX107815; RRID: AB_2036157 Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb Cell Signaling Technology Cat# 3108; RRID: AB_490941 Smad2 (D43B4) XP Rabbit mAb Cell Signaling Technology Cat# 5339; RRID: AB_10626777 IGF-I Receptor b (D23H3) XP Rabbit mAb Cell Signaling Technology Cat# 9750; RRID: AB_10950969 Cyclin D2 (D52F9) Rabbit mAb Cell Signaling Technology Cat# 3741; RRID: AB_2070685 c-Cbl (C49H8) Rabbit mAb Cell Signaling Technology Cat# 2179; RRID: AB_823449 HER4/ErbB4 (111B2) Rabbit mAb Cell Signaling Technology Cat# 4795; RRID: AB_2099883 Rac1/2/3 Antibody Cell Signaling Technology Cat# 2465; RRID: AB_2176152 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat# 5174; RRID: AB_10622025 Mouse anti-alpha-Tubulin (clone B-5-1-2) Sigma Cat# T5168; RRID: AB_477579 HRP Rabbit anti-Mouse (polyclonal) Abcam Cat# ab6728; RRID: AB_955440 HRP Goat anti-Rabbit (polyclonal) Abcam Cat# ab6721; RRID: AB_955447 Biological samples Normal and tumor tissue from cancer patients from different entities University Hospital Heidelberg or Dresden N/A Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Thermo Fisher Scientific Cat# 12634028 Glucose Thermo Fisher Scientific Cat# 15023021 L-glutamine Thermo Fisher Scientific Cat# 25030024 HEPES Sigma-Aldrich Cat# H0887 Heparin Sigma-Aldrich Cat# H3149 BSA PAN-Biotech Cat# P06-10200 EGF, recombinant human R&D Systems Cat# 236-EG FGF basic, recombinant human R&D Systems Cat# 233-FB TGF beta 1, recombinant human Immunotools Cat# 11343160 Cultrex Reduced Growth Factor Basement Membrane Extract R&D Systems Cat# 3433-010-01 B-27TM Supplement Thermo Fisher Scientific Cat# A3582801 Penicillin-Streptomycin (10.000 U/ml) Thermo Fisher Scientific Cat# 15140148 Nicotinamide Sigma-Aldrich Cat# 72340-100G N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250-25G SB 202190 Sigma-Aldrich Cat# S7067-5MG A 83-01 Sigma-Aldrich Cat# SML0788-5MG Gastrin Sigma-Aldrich Cat# SCP0152-1MG Prostaglandin E2 Sigma-Aldrich Cat# P5640-1MG Primocin InvivoGen Cat# ant-pm-1 Y-27632 Stem Cell Technologies Cat# 72302 RPMI1640 with GlutaMax Thermo Fisher Scientific Cat# 61870036 FBS PAN-Biotech Cat# P40-47500 DMSO Sigma-Aldrich Cat# 472301 PBS Life Technologies Cat# 14190169 0.9% NaCl solution B.Braun Cat# 3570350 (Continued on next page) e1 Cell Reports Medicine 4, 101200, October 17, 2023

Techniques: Sequencing, Expressing